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KMID : 1140120120170010056
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Cancer Prevention Research
2012 Volume.17 No. 1 p.56 ~ p.61
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Anti-Inflammatory Effect of Bitter Melon (Momordica charantia) in RAW 264.7 Cell
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Sin Seung-Mi
Mok So-Youn
Lee Sang-Hyun
Cho Kye-man
Cho Eun-Ju
Kim Hyun-Young
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Abstract
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We investigated the anti-inflammatory activity of bitter melon (Momordica charantia L.) in this study. Bitter melon was extracted with methanol and then fractionated into 4 fractions (hexane, methylene chloride, ethyl acetate, and butanol fractions). The extract and fractions from bitter melon showed the strong scavenging activities of nitric oxide (NO). Also, in order to investigate the anti-inflammatory activity of bitter melon, we employed NO-induced cellular inflammatory system using RAW 264.7 macrophage cell. Among the extract and fractions, MeOH extract and butanol fraction showed the highest protective effect from inflammation through increase in cell viability. In addition, bitter melon scavenged NO generated by both exotoxin SNP and endotoxin lipopolysaccharide (LPS) & interferon-gamma (IFN-γ). The results represent that bitter melon would have the protect role against inflammation induced by exotoxin and endotoxin. In particular, among the extract and fractions, the butanol fraction showed the highest protective effect from inflammation, suggesting that the butanol fraction from bitter melon includes the promising protective compounds from inflammation.
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KEYWORD
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Bitter melon (Momordica charantia L.), NO, Inflammation, RAW 264.7 cell, SNP, LPS & IFN-γ
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